How can the CRISPR system be utilized as an innovative approach to combat antibiotic resistance by specifically targeting efflux pumps in bacteria?
DOI:
https://doi.org/10.58445/rars.867Keywords:
CRISPR, antibiotic resistance, efflux pumpsAbstract
Antibiotic resistance has become a significant threat to global public health, rendering conventional treatments ineffective against bacterial infections. Efflux pumps, which actively expel antibiotics from bacterial cells, play a pivotal role in mediating this resistance. This review paper aims to explore the viability of utilizing CRISPR/Cas9 to disrupt efflux pumps in bacterial pathogens, potentially controlling antibiotic resistance. In our paper, we critically evaluate the current state of knowledge and future prospects of antibiotic resistance. We seek to shed light on the promising avenue of precision gene editing in the fight against antibiotic-resistant bacteria. We will go over three different papers written about the use of CRISPR in bacteria and its relation to efflux pumps. 2 of the papers will investigate how CRISPR/cas9 or CRISPRi can be used to lessen the effects of efflux pumps, and one of the papers will look at the natural CRISPR system in a bacteria and how it affects antibiotic resistance. Ultimately, this research paper aims to offer valuable insights and promote innovative strategies to effectively mitigate the global prevalent threat of antibiotic resistance.
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