Designing a prime editing guide to target the rs1190870 SNP associated with the development of adolescent idiopathic scoliosis in East Asian populations
DOI:
https://doi.org/10.58445/rars.3091Keywords:
Adolescent Idiopathic Scoliosis, Ladybird Homeobox 1, East Asian Populations, Crispr Prime Editing, Adeno-associated Virus, Single-nucleotide PolymorphismsAbstract
Several studies have reported the association of the Ladybird Homeobox 1 (LBX1) gene with the development of adolescent idiopathic scoliosis (AIS) in East Asian populations. AIS is a type of scoliosis that develops in adolescents with no definite cause and leads to spinal discomfort and complications. Within the regulatory regions of LBX1, there are single-nucleotide polymorphisms (SNPs) that are linked to susceptibility to AIS, specifically if an individual inherits what is deemed the risk allele. This paper discusses three SNPs that are linked to AIS within the East Asian population: rs11190870, rs678741, and rs625039. We then dive into the most highly associated SNP – rs11190870 – and design a prime editing guide utilizing the PegFinder website to edit the risk allele T to the non-risk allele A. This paper then discusses the optimal way to deliver the CRISPR complex in vivo, through an adeno-associated virus (AAV) directly into skeletal muscle cells. Our study proposes a CRISPR guide to replace a well-studied risk allele, potentially reducing the risk of developing scoliosis, utilizing cutting-edge gene technology that may benefit families with a history of scoliosis.
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