Preprint / Version 1

Assessing the Impact of Endocrine-Disrupting Chemicals in Makeup Products on Female Reproductive Health: Exposure Levels and Regulatory Improvements

##article.authors##

  • Jade Gounaris The Shipley School

DOI:

https://doi.org/10.58445/rars.2810

Keywords:

Endocrine-disrupting chemicals (EDCs), Cosmetic toxicology

Abstract

This report discusses the common endocrine disrupting chemicals present in makeup products and their effects on female reproductive health. It also reviews original data collected to understand the prevalence of these identified chemicals in the products of popular makeup brands. The chemicals surveyed for were lead, butylated compounds, phthalates, parabens, formaldehyde and formaldehyde-releasing preservatives, titanium dioxide, and per and polyfluoroalkyl substances. Three types of makeup products (lip gloss, concealer, and mascara) from both affordable (L’Oreal Paris, Pacifica Beauty, and Revlon) and luxury (Anastasia Beverly Hills, Dior Beauty, and Benefit Cosmetics) brands were surveyed for the identified endocrine disrupting chemicals using the Environmental Working Group database. 133 products were surveyed in total with every identified chemical except per or polyfluoroalkyl substances being present in at least 1 product. Since the United States does not regulate ingredients in cosmetic products as strictly as the European Union, more stringent regulations should be put in place. Public education and outreach should also be conducted to spread awareness to makeup consumers, especially young girls, on the importance of avoiding the use of endocrine-disrupting chemicals.

References

Alvarez, S. (2024, November 12). The 20 best luxury makeup products that are truly worth your

money. Glamour. https://www.glamour.com/gallery/beauty-awards-makeup-winners

Amiri, A., Pryor, E., Rice, M., Downs, C. A., Turner-Henson, A., & Fanucchi, M. V. (2015).

Formaldehyde exposure during pregnancy. MCN: The American Journal of Maternal/Child Nursing, 40(3), 180-185. https://pubmed.ncbi.nlm.nih.gov/25919211/

American Society for Reproductive Medicine. (2021). Disparities in access to effective treatment

for infertility in the United States: An ethics committee opinion. https://www.asrm.org/practice-guidance/ethics-opinions/disparities-in-access-to-effective-treatment-for-infertility-in-the-united-states-an-ethics-committee-opinion-2021/

California Department of Public Health. (2023, February). Formaldehyde.

https://www.cdph.ca.gov/Programs/CCDPHP/DEODC/OHB/HESIS/CDPH%20Document%20Library/formaldehyde.pdf

Campaign for Safe Cosmetics. (2022, July 7). Butylated compounds.

https://www.safecosmetics.org/chemicals/butylated-compounds/

Campaign for Safe Cosmetics. (2022, July 7). Lead and other heavy metals.

https://www.safecosmetics.org/chemicals/lead-and-other-heavy-metals/

Campaign for Safe Cosmetics. (2022, April 21). Phthalates.

https://www.safecosmetics.org/chemicals/phthalates/

Chemical Safety Facts. (2024a, March 12). Titanium dioxide.

https://www.chemicalsafetyfacts.org/chemicals/titanium-dioxide/

Cleveland Clinic (2022, August 11). Diminished Ovarian Reserve

my.clevelandclinic.org/health/diseases/23975-diminished-ovarian-reserve.

Cleveland Clinic. (2024, December 19). Endocrine system.

https://my.clevelandclinic.org/health/body/21201-endocrine-system

Cleveland Clinic. (2025, February 7). Infertility: Causes & treatment.

https://my.clevelandclinic.org/health/diseases/16083-infertility

Endocrine Society. (2019, November 20). Impact of EDCs on Reproductive Systems.

https://www.endocrine.org/topics/edc/what-edcs-are/common-edcs/reproduction

Environmental Protection Agency. (2024, November 26). Our current understanding of the

human health and environmental risks of PFAS. https://www.epa.gov/pfas/our-current-understanding-human-health-and-environmental-risks-pfas

Environmental Working Group. (n.d.). EWG Skin Deep® | What is butylene glycol.

https://www.ewg.org/skindeep/ingredients/700861-BUTYLENE_GLYCOL/

Environmental Working Group. (2025). Understanding Skin Deep® ratings || Skin Deep®

cosmetics database. https://www.ewg.org/skindeep/understanding_skin_deep_ratings/

European Commission (n.d.). Internal Market, Industry, Entrepreneurship and SMEs-

Legislation.

https://single-market-economy.ec.europa.eu/sectors/cosmetics/legislation_en

FDA. (2024, January 22). Per and polyfluoroalkyl substances (PFAS) in cosmetics. U.S. Food

and Drug Administration. https://www.fda.gov/cosmetics/cosmetic-ingredients/and-polyfluoroalkyl-substances-pfas-cosmetics

Gore, A. C., Chappell, V. A., Fenton, S. E., Flaws, J. A., Nadal, A., Prins, G. S., Toppari, J., &

Zoeller, R. T. (2015). EDC-2: The Endocrine Society’s second scientific statement on endocrine-disrupting chemicals. Endocrine Reviews, 36(6), E1–E150. https://doi.org/10.1210/er.2015-1010

Guardian News and Media. (2023, April 6). “Forever chemicals” linked to infertility in women,

study shows. The Guardian. https://www.theguardian.com/environment/2023/apr/06/forever-chemicals-infertility-women-pfas-blood

Gunderson, M. P., Roberge, M., Setchell, K. D., Boyd, G. R., Baird, D. D., Missmer, S. A.,

Waterland, R. A., Newbold, R. R., Farethold, D. A., Bergeron, J. M., Gale, R. W., Hayes, T. B., Wiig, O., Backlin, B. M., Adams, N. R., Smith, J. F., Mirocha, C. J., & Silva, M. J. (2006). Endocrine disrupters and female reproductive health. Best Practice & Research Clinical Endocrinology & Metabolism, 20(1), 63–82. https://doi.org/10.1016/j.beem.2005.09.007

Hao, Y., Wang, Y., Yan, L., Xu, X., Chen, D., Zhao, Y., & Qiao, J. (2023, June 2). Niehs Home

Page. National Institute of Environmental Health Sciences. https://www.niehs.nih.gov/

Insogna, I. G., & Ginsburg, E. S. (2018). Infertility, inequality, and how lack of insurance

coverage compromises reproductive autonomy. Journal of Ethics | American Medical Association. https://journalofethics.ama-assn.org/article/infertility-inequality-and-how-lack-insurance-coverage-compromises-reproductive-autonomy/2018-12

Jeong, S. H., Kim, B. Y., Kang, H. G., Ku, H. O., & Cho, J. H. (2005). Effects of butylated

hydroxyanisole on the development and functions of reproductive system in rats. Toxicology, 208(1), 49–62. https://doi.org/10.1016/j.tox.2004.11.013

Karimipour, M., Javanmard, M. Z., Ahmadi, A., & Jafari, A. (2018). Oral administration of

titanium dioxide nanoparticle through ovarian tissue alterations impairs mice embryonic development. International Journal of Reproductive BioMedicine, 16(6), 397–404. https://doi.org/10.22074/ijrm.2018.6079309

Land, K. L., Ghuneim, S. M., Williams, B. A., & Hannon, P. R. (2025). Phthalates disrupt female

reproductive health: a call for enhanced investigation into mixtures. Reproduction (Cambridge, England), 169(2), e240117. https://pmc.ncbi.nlm.nih.gov/articles/PMC11969576/

Mekdeci, D. (2020, March 12). Formaldehyde - birth defect fact sheet. Birth Defect Research for

Children. https://birthdefects.org/formaldehyde/

Million Marker Staff. (2024, August 26). Formaldehyde cosmetics uses: Side effects and healthy

alternatives. https://millionmarker.com/blogs/blog/skin-care-products-with-formaldehyde

Minghui, F., Ran, S., Yuxue, J., & Minjia, S. (2023). Toxic effects of titanium dioxide

nanoparticles on reproduction in mammals. Frontiers in Bioengineering and Biotechnology, 11, 1183592. https://doi.org/10.3389/fbioe.2023.1183592

National Cancer Institute. (n.d). NCI Dictionary of Cancer Terms.

https://www.cancer.gov/publications/dictionaries/cancer-terms/def/ovarian-reserve

Nugent, C. N., & Chandra, A. (2024). Infertility and impaired fecundity in women and men in the

United States, 2015–2019 (National Health Statistics Reports, No. 202). National Center for Health Statistics. https://dx.doi.org/10.15620/cdc/147886

Orth, T. (2023, December 11). How often — and why — American women wear makeup.

YouGov. https://today.yougov.com/society/articles/48130-why-american-women-wear-makeup-poll

Patel, S., Zhou, C., Rattan, S., & Flaws, J. A. (2015). Bisphenol A and reproductive health:

Update of experimental and human evidence, 2007–2013. Biology of Reproduction. https://doi.org/10.1095/biolreprod.115.130336

PlusChem. (2023, April 8). Differences in cosmetic regulations between the EU and U.S /

pluschem. https://pluschem.com/blog/eu-and-us-cosmetic-regulations/

Pop, A., Kiss, B., & Loghin, F. (2013). Endocrine disrupting effects of butylated hydroxyanisole

(BHA - E320). Clujul Medical (1957), 86(1), 15–19. https://doi.org/10.15386/cjmed-446

Qu, R., Wang, J., Li, X., Zhang, Y., Yin, T., & Yang, P. (2024). Per- and polyfluoroalkyl

substances (PFAS) affect female reproductive health: Epidemiological evidence and underlying mechanisms. Toxics, 12(9), 678. https://doi.org/10.3390/toxics12090678

Redox Medical Group. (2024, December 24).

Clean beauty - full list of parabens, sulfates, Phthalate and other harsh chemicals to avoid. Redox Medical Group. https://redoxmedicalgroup.com/clean-beauty-full-list-of-parabens-sulfates-and-phthalate-to-avoid/ s

Robin, M. (2024, November 12). 20 Best Drugstore & Affordable Makeup

Products of 2024 | Beauty Awards. Glamour. https://www.glamour.com/gallery/beauty-awards-drugstore-makeup-product-winners

Segal, T. R., & Giudice, L. C. (2019). Before the beginning: Environmental exposures and

reproductive and obstetrical outcomes. Fertility and Sterility, 112(4), 613–621. https://doi.org/10.1016/j.fertnstert.2019.08.012

Sharma, A., & Shrivastava, D. (2022). Psychological problems related to infertility. Cureus.

https://doi.org/10.7759/cureus.29516

Simionescu, G., Doroftei, B., Maftei, R., Obreja, B. E., Anton, E., Grab, D., ... & Anton, C.

(2021). The complex relationship between infertility and psychological distress. Experimental and Therapeutic Medicine, 21(4), 306.

Stoiber, T. (2019, April 9). What are parabens, and why don’t they belong in cosmetics?

Environmental Working Group. https://www.ewg.org/what-are-parabens

Tee-Melegrito, R. A. (2023, August 25). What is butylene glycol? Uses, benefits, and side

effects. Medical News Today. https://www.medicalnewstoday.com/articles/butylene-glycol

Toft, G., Jönsson, B. A., Lindh, C. H., Jensen, T. K., Hjollund, N. H., Vested, A., & Bonde, J. P.

(2012). Association between pregnancy loss and urinary phthalate levels around the time of conception. Environmental health perspectives, 120(3), 458-463.

https://ehp.niehs.nih.gov/doi/full/10.1289/ehp.1103552

U.S. Department of Health and Human Services. (2024, July 22). Endocrine disruptors. National

Institute of Environmental Health Sciences. https://www.niehs.nih.gov/health/topics/agents/endocrine

U.S. Department of Health and Human Services. (2023, June 29). PFAS exposure linked to

reduced fertility in women. National Institute of Environmental Health Sciences. https://www.niehs.nih.gov/research/supported/centers/core/spotlight/fertility

U.S. Food and Drug Administration. (n.d.). Cosmetics & U.S. law.

https://www.fda.gov/cosmetics/cosmetics-laws-regulations/cosmetics-us-law

U.S. Food and Drug Administration. (n.d.). Cosmetic ingredients.

https://www.fda.gov/cosmetics/cosmetic-products-ingredients/cosmetic-ingredients

Utah Fertility Center. (n.d). Guide to Antral Follicle Count.

https://utahfertility.com/post/guide-to-antral-follicle-count#:~:text=Antral%20follicles%20are%20small%2C%20fluid%2Dfilled%20sacs%20outside%20the%20ovaries,egg%20is%20released%20during%20ovulation.

Wang, L., Lou, Y., & Li, B. (2025). Exposure to titanium dioxide nanoparticles disrupts the BTB

by interfering with the assembly of stress granules in germ cells. Journal of Environmental Management, 373, 123799. https://doi.org/10.1016/j.jenvman.2024.123799

Wei, Y., Lin, Z., Huang, Q., Wu, H., Wang, R., & Wang, J. (2025). Burden of female infertility in

countries and territories, 1990–2021: results from the Global Burden of Disease Study 2021. Journal of Psychosomatic Obstetrics & Gynecology, 46(1). https://doi.org/10.1080/0167482X.2025.2459618

Weiss, M. S., & Marsh, E. E. (2023, October 1). Navigating unequal paths: Racial disparities in

the infertility journey. Obstetrics and gynecology. https://pmc.ncbi.nlm.nih.gov/articles/PMC10510808/

Wonnda (2024). The EU Cosmetics Regulation: Protecting Consumers and Promoting Quality.

EU Cosmetics Regulation Explained | Wonnda

World Health Organization. (2023, April 4). 1 in 6 people globally affected by infertility: WHO.

https://www.who.int/news/item/04-04-2023-1-in-6-people-globally-affected-by-infertility

World Health Organization. (2024, May 22). Infertility.

https://www.who.int/news-room/fact-sheets/detail/infertility

Posted

2025-07-27