Gene expression in bacterial pathogen against antibiotics
DOI:
https://doi.org/10.58445/rars.2116Keywords:
NDM1, antibiotics, antibiotic resistance, Real-time PCR, medicineAbstract
Carbapenem resistance among Enterobacteriaceae has emerged as a significant global antibiotic resistance issue. Identifying the resistance mechanisms in these clinically significant isolates is crucial for infection control and public health strategies. This study examined blood samples to detect the presence of the NDM-1 gene and qualitatively assessed its expression using real-time PCR. Our findings revealed that one sample tested positive for NDM-1, while another yielded inconclusive results. We discuss the implications of these findings and the study's limitations.
References
https://wwwnc.cdc.gov/eid/spotlight/antimicrobial-resistance
Delcour, A.H., 2009. Outer membrane permeability and antibiotic resistance. Biochimica et Biophysica Acta, 1794(5), pp.808-816. https://doi.org/10.1016/j.bbapap.2008.11.005
Rossolini, G.M., Arena, F. and Pollini, S., 2014. Novel infectious diseases and emerging Gram-positive multi-resistant pathogens in hospital and community-acquired infections. In: F. Marinelli and O. Genilloud, eds. Antimicrobials. Berlin: Springer. https://doi.org/10.1007/978-3-642-39968-8_2
Goossens, H. and Sprenger, M.J., 1998. Community-acquired infections and bacterial resistance. BMJ (Clinical research ed.), 317(7159), pp.654-657. https://doi.org/10.1136/bmj.317.7159.654
Thakkar, J., Shanoo, A., Gupta, S. and Thakkar, A., 2023. The pattern and impact of hospital-acquired infections and its outlook in India. Cureus, 15(11), e48583. https://doi.org/10.7759/cureus.48583
https://www.thelancet.com/journals/langlo/article/PIIS2214-109X(22)00317-5/fulltext. Murhekar, M.V. and Kumar, C.P.G., n.d. Health-care-associated infection surveillance in India. Indian Council of Medical Research—National Institute of Epidemiology, Chennai 600 077, India. https://doi.org/10.1016/S2214-109X(22)00317-5
Fomda, B.A., Khan, A. and Zahoor, D., 2014. NDM-1 (New Delhi metallo beta lactamase-1) producing Gram-negative bacilli: emergence & clinical implications. The Indian Journal of Medical Research, 140(5), pp.672-678.
Cornaglia, G., Akova, M., Amicosante, G., Cantón, R., Cauda, R., Docquier, J.D. and ESCMID Study Group for Antimicrobial Resistance Surveillance (ESGARS), 2007. Metallo-β-lactamases as emerging resistance determinants in Gram-negative pathogens: open issues. International Journal of Antimicrobial Agents, 29(4), pp.380-388.
Li, X., Zhao, D., Li, W., Sun, J., and Zhang, X., 2021. Enzyme inhibitors: The best strategy to tackle superbug NDM-1 and its variants. International Journal of Molecular Sciences, 23(1), p.197. https://doi.org/10.3390/ijms23010197
Laboratory Testing for Klebsiella pneumoniae Carbapenemase (KPC) and New Delhi metallo-β-lactamase (NDM) in Gram-negative Bacteria. https://www.cdc.gov/gram-negative-bacteria/php/laboratories/
Downloads
Posted
Categories
License
Copyright (c) 2025 Shyla Sama
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.