The Manipulation of Growth Factors and ECM to Optimize Patency and Endothelialization of iPSCs
DOI:
https://doi.org/10.58445/rars.1705Keywords:
ECM, IPSC, Growth Factors, Coronary Artery Bypass Graft, Cell Surface Markers, EndothelialAbstract
Coronary artery bypass graft surgeries are among the most common heart surgeries. While synthetic vascular grafts are used, autologous vascular grafts are favored due to the lack of immune rejection from the patient. However, current autologous vascular grafts primarily use direct tissue extraction placed onto arteries, which may lead to adverse long-term effects on the patient. Thus, in-vitro-derived vascular grafts are needed. One of the methods used to produce a vascular graft is iPSC-derived endothelial cells. However, although it is known that iPSCs may induce differentiation into endothelial cells using growth factors, the optimal conditions for doing so have yet to be understood. This proposal aims to identify the optimal combination as well as the sequence of growth factors and extracellular matrix signals to yield the greatest patency and differentiation of iPSCs into endothelial cells inside extracellular matrices.
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