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Investigating the Effect of Concentration of Sodium Chloride on the Activity for Trypsin Hydrolysis of Albumin

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  • Shi-Ting Yao Shanghai High School International Division

DOI:

https://doi.org/10.58445/rars.412

Abstract

The purpose of this paper is to present findings regarding the topic of enzyme activity and inhibitors. Since research on the enzyme trypsin is minimal, investigations were performed to understand the enzyme's activity and whether it differs from that of alpha-amylase. Results show that when the concentration of sodium chloride increases, the rate of trypsin activity decreases. Hence, salt takes the role of an inhibitor, blocking enzyme active sites and destabilizing its structure. The optimal concentration of sodium chloride is 1.0% for optimal enzyme performance apart from the control, showing that excessive salt intake could be fatal to health. 

References

Audipudi, A.V., Bhaskar, C.V.S., & Didla, M. (2017). “Effect of Argo residues on Amylase activity of Bacillus species.” International Journal of Current Microbiology and Applied Science, (5), 107-115. https://www.ijcmas.com/special/5/Amrutha%20 V .%20Audipudi3,%20et%20al.pdf

“CLEAPSS Home Page.” CLEAPSS Home Page, www.cleapss.org.uk/. Accessed 28 Aug. 2023.

Edulab. “6 Tips For Glassware Safety In Labs.” Edulab, https://edulab.com/x-tips-for- glassware-safety-in-labs/.

Kortt, A A. “The preparation of trypsins and chymotrypsins from bovine and porcine residues after insulin extraction.” Preparative biochemistry vol. 8,2-3 (1978): 195-214. doi:10.1080/00327487808069060

Łukowski,A.,&Dec,D.(2018).“InfluenceofZn,Cd,andCufractionsonenzymatic activity of arable soils.” Environmental monitoring and assessment. 190(5), 278. https://doi:10.1007/s10661-018-6651-1

Meneton, Pierre, et al. “Links between dietary salt intake, renal salt handling, blood pressure, and cardiovascular diseases.” Physiological Reviews, vol. 85, no. 2, 2005, pp. 679–715, https://doi.org/10.1152/physrev.00056.2003.

Olsen, Jesper V et al. “Trypsin cleaves exclusively C-terminal to arginine and lysine residues.” Molecular & cellular proteomics : MCP vol. 3,6 (2004): 608-14. doi:10.1074/mcp.T400003-MCP200

Polgár, L. The catalytic triad of serine peptidases. Cell. Mol. Life Sci. 62, 2161–2172 (2005). https://doi.org/10.1007/s00018-005-5160-x

Shen, Chang-Hui. “Quantification and analysis of proteins.” Diagnostic Molecular Biology, 2019, pp. 187–214, https://doi.org/10.1016/b978-0-12-802823-0.00008-0.

Sinha, R., & Khare, S.K. (2014). “Protective role of salt in catalysis and maintaining structure of halophilic proteins against denaturation.” Frontiers in microbiology, 5, 165. https://doi.org/10.3389/fmicb.2014.00165

Wang, Chao et al. “High-Salt Diet Has a Certain Impact on Protein Digestion and Gut Microbiota: A Sequencing and Proteome Combined Study.” Frontiers in microbiology vol. 8 1838. 21 Sep. 2017, doi:10.3389/fmicb.2017.01838

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Posted

2023-10-02